Ilya Kolb, PhD

Ilya Kolb, PhD

Biomedical engineer with experience in high-throughput screening, biomedical automation, microscopy, and open-source software. Platform-independent problem solver.

PatcherBot: walk-away automated patch-clamp robotics

About

  • Enables automated patch-clamp recordings in cultured cells, brain slices and in vivo
  • Autonomously performs experiments for 3+ hours with no human intervention
  • Uses computer vision and automated pipette cleaning to close the loop

Demonstration video

Two pipettes independently patch-clamp multiple HEK cells on a glass cover slip sequentially without human intervention over the span of 27 minutes. Green light indicates successful whole-cell recording.

Other patcherBot videos

[Timelapse experiment] [In vivo experiment] [Single-pipette experiment]

Details

For more details on implementation, see the PatcherBot paper and the pipette cleaning paper